The assessment was based on an examination of the remnants of the weapon used in the 25 August airstrike.
The Saudi Arabia-led military coalition admitted carrying out the attack, blaming civilian casualties on a “technical error”.
Lynn Maalouf, Amnesty International’s Middle East research director, said: “We can now conclusively say that the bomb that killed Buthaina’s parents and siblings, and other civilians, was made in the USA.
Yemen has been since March 2015 under a brutal aggression by Saudi-led coalition. Tens of thousands of Yemenis have been injured or martyred in Saudi-led strikes, with the vast majority of them are civilians.
The coalition has been also imposing a blockade on the impoverished country’s ports and airports as a part of his aggression which is aimed at restoring power to fugitive former president Abdrabbuh Mansour Hadi.